Amino acid sequence of rat kidney glutathione synthetase.

Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.

Glutathione S-transferase rGSTM5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. The protein was digested with Achromobacter protease I or endoproteinase Arg-C. The peptide fragments were isolated for electrospray MS and N-terminal peptide sequencing analyses. The primary amino acid sequence of rGSTM5* comprises 217 residu...

Identification of bile acid coenzyme A synthetase in rat kidney.

Bile acid CoA synthetase has been discovered in rat kidney. Incubation of kidney microsomes with [14C]chenodeoxycholic acid and CoA produced a single peak with the high performance liquid chromatography (HPLC) retention time of CDC-CoA. This peak, when incubated with purified bile acid CoA: amino acid N-acyltransferase (BAT) from human liver and either taurine or glycine, led to the formation o...

Amphibian embryo glutathione transferase: amino acid sequence and structural properties.

Complete amino acid sequence of pig kidney fructose-1,6-bisphosphatase.

The covalent structure of the pig kidney fructose-1,6-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 3.1.3.11) subunit has been determined. Placement of the 335 amino acid residues in the polypeptide chain was based largely on automated Edman degradation of eight purified cyanogen bromide fragments generated from the S-carboxymethylated protein. The determination of the amin...

Amino acid sequence of Escherichia coli glutamine synthetase deduced from the DNA nucleotide sequence.

Glutamine synthetase is encoded by the glnA gene of Escherichia coli and catalyzes the formation of glutamine from ATP, glutamate, and ammonia. A 1922-base pair fragment from a cDNA containing the glnA structural gene for E. coli glutamine synthetase has been sequenced. An open reading frame of 1404 base pairs encodes a protein of 468 amino acid residues with a calculated molecular weight of 51...